Primordial germ cell (PGC) specification occurs early in development.PGC specifiers have been identified in Drosophila, mouse, and human but remained elusive in most animals.Here we identify the RNA-binding protein Dnd as a critical Soccer - Goalie - Clothing PGC specifier in the medaka fish (Oryzias latipes).Dnd depletion specifically abolished PG
Summary: Off-target binding and cleavage by Cas9 pose major challenges in its application.How the conformational dynamics of Cas9 govern its nuclease activity under on- and off-target conditions remains largely unknown.Here, using intra-molecular single-molecule fluorescence resonance energy transfer measurements, we revealed that Cas9 in apo, sgRN
The article is devoted to the philosophical understanding of the problem of perception of values and antivalues.Beliefs, recognized among the people regarding the purposes to which it is important to strive for life, named values.They are the Foundation for moral principles and provide people with their human existence despite the fact that differe
The timely and accurate recognition of damage to buildings after destructive disasters is one of the most important post-event responses.Due to the complex and dangerous situations in affected areas, field surveys of post-disaster conditions are not always feasible.The use of satellite imagery for disaster assessment can overcome this problem.Howev
Direct-coupled cavity bandpass filters with Chebyshev response are studied and realized, using the Door Hinge Shim technique of planar substrate-integrated waveguide (SIW) for the purpose of high-density integration and cost reduction.Using a general mode-matching technique, the scattering matrix of waveguide bifurcation-type discontinuity and asym